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In vitro cytotoxicity of PEG-PEI and PPSR in primary microglial cells determined by an MTT assay. (a) Primary microglial cells treated with PEG-PEI at various concentrations from 0 to 200 μg/ml. (b) Primary microglial cells treated with PPSR at various N/P ratios from 0 to 100. The dose of PPSR was 100 nM in each well and incubation time was 24 h. Values are mean ± SD (n = 3). ***P < 0.001 vs. control. PPSR, polyethyleneglycol-polyethyleneimine deliver ROCK2-siRNA;.
