Fig. 2.

Gene-silencing effect of PPSR in primary microglial cells. (a) The gene-silencing effect of PPSR (100 nM) in primary microglial cells was detected by RT-PCR. (b) The gene-silencing effect of PPSR (100 nM) in primary microglial cells was detected by western blotting. (c) The relative protein expression levels of ROCK2 in each group. The control was treated with PBS. Values are mean ± SD, ***P < 0.001, compared with the control. PPSR, polyethyleneglycol-polyethyleneimine deliver ROCK2-siRNA; RT-PCR, reverse transcription-PCR