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. 2021 Dec 17;12:775508. doi: 10.3389/fpls.2021.775508

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Copyright © 2021 Zhang, Wang, Chen, Wu, Feng and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Editing of the GmLMM1 causes the resistance to M. incognita. (A) Editing of GmLMM1 by CRISPR/Cas9. The CRISPR target sequence is shown in orange, and the corresponding protospacer adjacent motif (PAM) site is shown in blue. The box indicates the putative sites shear cut by cas9. (B) Phenotype appearance of the line #9 mutant root. Scale bar presents 1 cm. (C) M. incognita parasites numbers per gram of soybean roots at 7 dpi. (D,E) M. incognita gall (D) and female (E) numbers per gram of soybean roots at 30 dpi. At least three biological replicates of (C–E) were performed, and the similar results were obtained. Data are presented as the mean ± S.D., n ≥ 10; Student's t-test.