FIGURE 2.

Effect of 18KHT01 on differentiation of 3T3-L1 adipocytes. The 3T3-L1 pre-adipocytes were seeded in separate plates. The cells of one plate were treated with samples at (A) Day 0 by mixing with MDI cocktail and another at (B) Day 2 with insulin media. (C) The 18KHT01 and its ingredients at an equal concentration of 80 μg/ml were separately treated to evaluate synergistic effect. The amounts of lipid accumulations were quantified by Oil red O (ORO) assay. (D) The lipid accumulation in the adipocytes was visualized before and after ORO staining using a light microscope and images were captured at ×20 magnification. Statistical significance was calculated using one-way ANOVA followed by Dunnett’s multiple comparisons test. The data shown are represented as mean ± SD of four separate experiments with significance ***p < 0.001 vs control. NC = Negative control; Control = MDI control (Blank treated); EGCG = (-)-Epigallocatechin-3-gallate (100 µM); CS = Camellia sinensis; QA = Quercus acutissima; GT = Geranium thunbergii; Lemon = Citrus limon.