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Characterization of peptide AEDPPE. (A) AEDPPE is located at amino acids 100 to 109 of heat-shock protein beta-1 (HSPB1). (B) Chemical structure of AEDPPE. (C) Mass spectral analysis of chemically synthesized AEDPPE. (D) HTR-8/SVneo cells were incubated with FITC-AEDPPE (green) for 1 h and then stained with DAPI (blue). AEDPPE was widely distributed throughout the cytoplasm and nucleus after penetrating the cell membrane (scale bar = 100 μm).
