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. 2021 Dec 17;8:738378. doi: 10.3389/fcvm.2021.738378

Figure 3.

Figure 3

AEDPPE ameliorates TNF-α-induced dysfunction in HTR-8/SVneo cells. (A) Treatment of HTR-8/SVneo cells with TNF-α or AEDPPE+TNF-α did not significantly affect cellular proliferation. (B) Stimulation with TNF-α and AEDPPE did not affect HTR-8/SVneo cell apoptosis. (C) AEDPPE significantly reduced TNF-α-induced mRNA expression of IL-1β, IL-6, IL-8, and IL-6 in cellular supernatants. (D) Effects of TNF-α and TNF-α+AEDPPE on mRNA expression of sFlt-1 and PlGF in HTR-8/SVneo cells, and concentrations of sFlt-1 and PlGF protein in cellular supernatant. (E) AEDPPE significantly improved wound healing in HTR-8/SVneo cells after TNF-α injury (scale bar = 400 μm). (F) AEDPPE promoted migration and invasion of HTR-8/SVneo cells even after TNF-α injury (scale bar = 200 μm). (G,H) Effects of TNF-α and TNF-α+AEDPPE on mRNA and protein expression of MMP-2. *p < 0.05, **p < 0.01, ***p < 0.001. IL, interleukin; PlGF, placental growth factor; sFlt-1, soluble FMS-like tyrosine kinase 1; TNF-α, tumor-necrosis factor alpha.