FIGURE 2.

AMG5 co-expresses VAChT/ChAT and Ebf. (A) An Ebf reporter plasmid labels various CNS neurons including AMG5 at 18 h post-fertilization (hpf) at 20°C. Image is a confocal Z stack projection. (B) Co-electroporation of the Ebf reporter and the minimal AMG5-specific VAChT/ChAT reporter revealed co-expression in AMG5 at 17 hpf, 20°C. In this example, the larva shows mosaic incorporation of the plasmids only in the animal pole-derived lineages, which give rise to AMG5 and the Bipolar Tail Neuron (BTN) that also expresses Ebf (Stolfi et al., 2015), but not the core MG.