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WASP KO HL-60 cells exhibit relatively minor migration defects on flat substrates (as in Fig. 7, C and G–I, top). Cells are labeled with NucBlue for tracking and imaged for 1 h at 37°C in 5% CO2. Overlaid are cell trajectories that last at least half the movie. Track color represents frame number. Scale bar is 10 μm. Time, min:s. Video is displayed at 10 fps.
