Figure S4.

Neutrophil DREAM positively regulates ROS production in neutrophils stimulated with TNF-α but not fMLP. WT and DREAM KO neutrophils were adhered to Fg-coated surfaces and then incubated with luminol and horseradish peroxidase. After the addition of 20 ng/ml TNF-α or 10 µM fMLP, light emission was recorded every 20 s at 37°C for 40 min. (A and C) A representative trace of ROS production. RLU, relative light unit. (B and D) The quantification graph is shown as area under the curve (AUC) of RLU. Mean ± SD (n = 3). *, P < 0.05 versus WT, Student’s t test.