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. 2021 Nov 9;219(1):e20211083. doi: 10.1084/jem.20211083

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© 2021 Li et al.

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Figure S5. — Genotyping and complete blood counts of SCD mice and nonhematopoietic and/or hematopoietic DREAM-deficient SCD mice. SCD (Hbb^−/−) mice deficient in nonhematopoietic and/or hematopoietic DREAM were generated by transplantation of BM cells from SCD mice and DREAM-deficient SCD mice into irradiated WT and DREAM KO mice. (A and B) SCD and DREAM KO SCD mice were verified by PCR and RT-qPCR. (C–H) Blood cells from mice were counted (mean ± SD, n = 6 mice per group). WBC, white blood cells; NE, neutrophils; LY, lymphocytes; MO, monocytes; PLT, platelets. *, P < 0.05; **, P < 0.01; ***, P < 0.001 after ANOVA and Tukey’s test. FWD, forward; REV, reverse; Mut, mutant (Hbb⁻); Tg, transgene.