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. 2021 Dec 17;9:802502. doi: 10.3389/fcell.2021.802502

FIGURE 3.

FIGURE 3

NEU1 inhibitor modulated autophagy, mitochondrial fission, and mitophagy in DOX-treated rat hearts. (A) Representative images of LC3II expression in heart tissues of the three groups (scale bar = 50 μm). (B) The expression levels of LC3I/II, ATG5, Beclin 1, and P62 in heart tissues of the different groups by western blot (n = 6 per group). (C) Representative electron microscopy images of heart tissues in the three groups; the red arrows direct autophagic vacuoles containing cargos (scale bar = 1 μm). (D) The expression levels of dynamin-related protein 1 (Drp1), PINK1, and Parkin in heart tissues of the three groups by western blot (n = 6 per group). (E) Representative immunohistochemical images of Drp1, PINK1, and Parkin expressions in heart tissues of the three groups (n = 5 per group, scale bar = 50 μm). (**p < 0.01 vs. control group, ***p < 0.001 vs. control group. #p < 0.05 vs. DOX group, ##p < 0.01 vs. DOX group, ###p < 0.001 vs. DOX group).