Table 2. Immunogenetic characteristics of isolated envelope (Env)-reactive mAbs of Env-vaccinated infant monkeys based on rhesus macaque immunoglobulin database analysis.
| Animal ID | Group | Tissue | IgH ID | VH gene | DH gene | JH gene | HC % SHM | HC CDR3 length | Ig Isotype | IgL ID | VL/VK gene | JL/JK gene | Specificity |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 45521 | HIV Env+adjuvant | Spleen | H691207 | IGHV4-j*02 | IGHD3-9*01 | IGHJ5-2*01 | 3.82 | 23 | IgG | K690414 | IGKV1-n*01 | IGKJ2-1*01 | Undetermined |
| 45521 | HIV Env+adjuvant | Retropharyngeal LN | H691248 | IGHV4-j*02 | IGHD3-9*01 | IGHJ5-2*01 | 5.21 | 23 | IgA | K690428 | IGKV1-n*01 | IGKJ2-1*01 | Undetermined |
| 45521 | HIV Env+adjuvant | Retropharyngeal LN | H691255 | IGHV4-j*03 | IGHD3-9*01 | IGHJ5-2*01 | 4.51 | 23 | IgG | K690431 | IGKV1-n*01 | IGKJ2-1*01 | Undetermined |
| 45521 | HIV Env+adjuvant | Retropharyngeal LN | H691256 | IGHV4-j*02 | IGHD3-9*01 | IGHJ5-2*01 | 3.82 | 23 | IgG | K690432 | IGKV1-n*01 | IGKJ2-1*01 | Undetermined |
| 45522 | HIV Env+adjuvant | Spleen | H691308 | IGHV4-n*01 | IGHD6-24*01 | IGHJ4*01 | 7.90 | 13 | IgG | L690936 | IGLV1-e*01 | IGLJ3*01 | Undetermined |
| 45083 | Coadministration | Spleen | H691279 | IGHV4-f*02 | IGHD6-34*01 | IGHJ4*01 | 7.56 | 13 | IgM | L690918 | IGLV1-b*01 | IGLJ3*01 | V1V2 |
| 45083 | Coadministration | Mediastinal LN | H691285 | IGHV4-g*02 | IGHD3-26*01 | IGHJ4*01 | 4.17 | 17 | IgG | K690448 | IGKV1-a*01 | IGKJ2-1*01 | V1V2 |
| 45083 | Coadministration | Mediastinal LN | H691285 | IGHV4-g*02 | IGHD3-26*01 | IGHJ4*01 | 4.17 | 17 | IgG | L690922 | IGLV2-i*01 | IGLJ1*01 | V3 |
| 45083 | Coadministration | Mediastinal LN | H691289 | IGHV4-f*03 | IGHD5-5*02 | IGHJ4*01 | 5.15 | 13 | IgG | L690925 | IGLV2-a*01 | IGLJ1*01 | Undetermined |
| 45091 | Coadministration | Axillary LN | H691299 | IGHV3-g*03 | IGHD4-4*02 | IGHJ6*01 | 6.80 | 14 | IgG | L690932 | IGLV11-a*01 | IGLJ2*03 | Undetermined |
| 45091 | Coadministration | Axillary LN | H691306 | IGHV4-j*02 | IGHD4-22*01 | IGHJ4*01 | 5.15 | 17 | IgG | K690454 | IGKV1-e*05 | IGKJ4-1*01 | V3 |
| 45091 | Coadministration | PBMC | H691004 | IGHV3-al*01 | IGHD3-3*01 | IGHJ4*01 | 7.99 | 19 | IgG | L690726 | IGLV3-c*02 | IGLJ2*03 | CD4 binding site |
| 45435 | Extended Interval | Mediastinal LN | H691315 | IGHV4-f*03 | IGHD3-21*01 | IGHJ3*01 | 7.22 | 18 | IgG | K690458 | IGKV1-f*04 | IGKJ4-1*01 | V1V2 |
| 45435 | Extended Interval | Mediastinal LN | H691312 | IGHV4-j*02 | IGHD1-39*01 | IGHJ5-1*01 | 5.56 | 16 | IgG | K690456 | IGKV1-n*01 | IGKJ2-1*01 | CD4 binding site |
| 45435 | Extended Interval | Mediastinal LN | H691321 | IGHV3-al*01 | IGHD3-14*01 | IGHJ6*01 | 2.43 | 17 | IgG | K690463 | IGKV1-r*01 | IGKJ2-1*01 | V1V2 |
| 45435 | Extended Interval | Mediastinal LN | H691322 | IGHV3-r*02 | IGHD6-29*01 | IGHJ6*01 | 2.43 | 12 | IgG | K690464 | IGKV3-c*01 | IGKJ1-1*01 | V1V2 |
| 45441 | Extended Interval | Spleen | H691341 | IGHV3-y*03 | IGHD4-22*01 | IGHJ6*01 | 4.86 | 0 | IgG | K690472 | IGKV1-a*01 | IGKJ1-1*01 | Undetermined |
| 45441 | Extended Interval | Spleen | H691346 | IGHV4-j*02 | IGHD3-21*01 | IGHJ4*01 | 13.75 | 0 | IgG | L690948 | IGLV1-e*01 | IGLJ7*01 | CD4 binding site |
| 45441 | Extended Interval | Spleen | H691343 | IGHV3-y*03 | IGHD2-25*01 | IGHJ1*01 | 5.21 | 15 | IgG | L690946 | IGLV2-j*16 | IGLJ6*01 | Undetermined |
| 45441 | Extended Interval | Submental LN | H691216 | IGHV4-j*02 | IGHD3-3*01 | IGHJ6*01 | 1.39 | 18 | IgA | K690417 | IGKV2-d*03 | IGKJ1-1*01 | Undetermined |
| 45851 | 3M-052-SE | PBMC | H680141 | IGHV3-l*02 | IGHD5-23*01 | IGHJ5-2*01 | 0.00 | 11 | IgG | K680049 | IGKV2-r*01 | IGKJ1-1*01 | Undetermined |
| 45851 | 3M-052-SE | PBMC | H680144 | IGHV4-m*02 | IGHD1-A*01 | IGHJ4*01 | 2.38 | 13 | IgG | K680048 | IGKV2-g*01 | IGKJ1-1*01 | Undetermined |
| 45851 | 3M-052-SE | PBMC | H680146 | IGHV4-f*02 | IGHD6-24*01 | IGHJ4*01 | 4.47 | 10 | IgD | L680105 | IGLV5-b*01 | IGLJ1*01 | V3 |
| 45851 | 3M-052-SE | PBMC | H680147 | IGHV5-b*02 | IGHD7-A*01 | IGHJ6*01 | 2.43 | 13 | IgG | L680103 | IGLV1-d*01 | IGLJ2*03 | V3 |
| 45851 | 3M-052-SE | PBMC | H680149 | IGHV4-f*02 | IGHD6-24*01 | IGHJ4*01 | 5.15 | 10 | IgE | L680109 | IGLV6-c*01 | IGLJ2*03 | V3 |
| 45851 | 3M-052-SE | PBMC | H680150 | IGHV4-m*02 | IGHD1-A*01 | IGHJ4*01 | 2.04 | 13 | IgG | L680108 | IGLV5-b*01 | IGLJ1*01 | V3 |
A total of 26 pairs of potentially Env-reactive mAbs were isolated from the four vaccination groups across several anatomic compartments. Frequency of gene usage, percent somatic hypermutation, and complementarity-region 3 (CDR3) length are displayed for the heavy and light chains for each mAb along with the isotype and epitope specificity.