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Purification of TbLysoPLA (A.) by affinity chromatography on GE glutathione sepharose 4B. Lanes F1-F4 correspond to the collected fraction after GST release by thrombin cleavage. Nickel affinity purification of TbGK-6xHis fusion protein (B.) on HisPur Ni-NTA resin. Lanes 1–4: washes; lanes 5–8: imidazole elution fractions.
