Skip to main content
. 2021 Sep 28;144(12):3674–3691. doi: 10.1093/brain/awab356

Figure 2.

Figure 2

PARIS Y137 phosphorylation by c-Abl is required for PARIS-induced cytotoxicity. (A) Trypan blue exclusion cell viability assessment in SH-SY5Y cells transfected with FLAG-PARIS and/or GFP-Bcr-Abl (Bcr-Abl, 48 h) with or without treatment with the c-Abl inhibitor STI-571 (10 µM, 44 h; n = 6 per group). (B) Trypan blue exclusion cell viability assessment in SH-SY5Y cells transfected with FLAG-PARIS or a phospho-deficient Y137F-PARIS mutant with or without GFP-Bcr-Abl (48 h; n = 6 per group). (C) Trypan blue exclusion cell viability assessment in SH-SY5Y cells transfected with FLAG-PARIS and/or GFP-Bcr-Abl (48 h) and treated with the poly (ADP-ribose) polymerase inhibitor 3AB (10 µM, 12 h) or pan-caspase inhibitor Z-VAD (100 µM, 12 h; n = 6 per group). (D) Representative TUNEL assay images of the ventral midbrain from mice that underwent stereotaxic nigral injection of AAV-Con or AAV-PARIS (3 weeks). The coronal brain sections were counterstained with DAPI. Quantification of the percentage of TUNEL-labelled cells in AAV-Con- or AAV-PARIS-injected ventral midbrain regions (right panel, n = 12 sections from three mice per group). Data are expressed as mean ± SEM. Statistical analysis consisted of a one-way ANOVA test followed by Tukey’s post hoc analysis or unpaired two-tailed Student’s t-test. *P < 0.05, **P < 0.01 and ***P < 0.001. n.s. = non-significant. WT = wild-type.