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. 2021 Nov 18;10:e71900. doi: 10.7554/eLife.71900

Figure 2. Comparison of phylogeny, S. scabies suppression (red color scales).

P. infestans suppression (green color scale), G. graminis pv. tritici (take-all) suppression (blue color scale), phenotypes (gray color scales), natural product biosynthetic gene clusters (filled circles = presence of a gene or gene cluster), and the accessory genome (separated from biosynthetic gene clusters [BGCs] by a dotted line). In the phylogenetic tree of Pseudomonas strains, blue strains were collected from irrigated plots while orange strains were collected from unirrigated plots. All other strains were collected from the pre-irrigation plots. Figure 2—figure supplement 3 shows the relationship between these strains and the Pseudomonas phylogenomic groups defined by Garrido-Sanz et al., 2016.

Figure 2.

Figure 2—figure supplement 1. Representative images of Pseudomonas phenotypes.

Figure 2—figure supplement 1.

(A) Motility; (B) UV siderophore secretion; (C) hydrogen cyanide (HCN) production; (D) protease production.
Figure 2—figure supplement 2. Representative images of on-plate suppressive activity of Pseudomonas isolates.

Figure 2—figure supplement 2.

(A) Versus S. scabies; (B) versus Gaeumannomyces graminis pv. tritici (take-all); (C) versus P. infestans.
Figure 2—figure supplement 3. Maximum likelihood tree of full-length gyrB nucleotide sequences of Pseudomonas strains with representatives of the eight Pseudomonas phylogenomic groups defined by Garrido-Sanz et al., 2016.

Figure 2—figure supplement 3.

Black names represent pre-planting isolates, blue names represent isolates from irrigated soil, and orange names indicate isolates from unirrigated soil. Bootstrap values are shown for each branch in orange text. Sequences were aligned with MUSCLE (Edgar, 2004) and the tree was inferred using RAxML (Stamatakis, 2014). The tree was visualized using iTOL (Letunic and Bork, 2016). An interactive version of the tree annotated with features shown in Figure 2 is available at: https://itol.embl.de/tree/902431818658671633339579.