Figure 6. The role of the Ps619 cyclic lipopeptide (CLP) and hydrogen cyanide (HCN) gene clusters in S. scabies suppression.
(A) Predicted structure of the tensin-like molecule and liquid chromatography–tandem mass spectrometry (LC-MS) analysis of CLP production in wild-type (WT) Ps619 and a mutant (Ps619 ∆ten) with an in-frame deletion of nonribosomal peptide synthetase (NRPS) gene 02963 (see Figure 4—figure supplement 3 for biosynthetic gene cluster [BGC] information). (B) Cross-streak assays of Ps619 and associated mutants with S. scabies. See Figure 6—figure supplement 2A for assays with drier plates. (C) Cryo-scanning electron microscopy (Cryo-SEM) images of the interfacial region between the Ps619 strains and S. scabies. The order of images is identical to the cross-streaks in panel (B).
Figure 6—figure supplement 1. Pearson correlation coefficients for phenotypes across both sequenced and unsequenced isolates.
Isolates where one or more reliable phenotypes were not obtained (Supplementary file 1) were omitted from this correlation analysis.
Figure 6—figure supplement 2. Effect of Ps619 on S. scabies growth and development.
(A) Cross-streak assays (7 days post-inoculation) of Ps619 and associated mutants with S. scabies on drier conditions than in Figure 6B. S. scabies (Ss) is streaked vertically first and then Ps619 is streaked horizontally. (B) Split plates (6 days post-inoculation) of S. scabies grown alongside Ps619 WT and mutant strains. The characteristic gray spore pigment of S. scabies (seen in control plate) is not present when grown alongside wild-type or Δten strains.
Figure 6—figure supplement 3. Further cryo-scanning electron microscopy (cryo-SEM) images of the interfacial region between the Ps619 strains and S. scabies (Ss) showing that a well-defined boundary only exists with the Ps619 ∆ten∆hcn strain (bottom right).
