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FosGFP+ cells are neurons. (A) FosGFP expression across six layers in barrel cortex from a naïve mouse that was unexposed to training cage. (Scale bar, 100 μm.) (B) Immunostaining of NeuN in barrel cortex L2/3 of brain sections from naïve fosGFP mice that were unexposed to training cage. (Scale bar, 50 μm.) (C) Fraction (mean ± SEM) of fosGFP+ cells out of NeuN+ cells and NeuN+ cells out of fosGFP+ cells. n = 3 mice.
