FIG. 1.

(A) CgCBF1 gene expression complements the methionine biosynthetic defect of an S. cerevisiae cbf1 null mutant strain. Growth of CC718–1A, CC718–1A/pYep#9.1, and CC718–1A/pRSCg1 on SD medium lacking methionine (met) is shown. The plates were incubated for 4 days at 30°C. (B) Genomic map of the cloned DNA insert in plasmid pYep#9.1. Major ORFs were detected and analyzed using BLAST and the Wisconsin Sequence Analysis software package (GCG9). The positions of the ORFs are indicated by arrows. Flanking thick lines represent vector sequences. Restriction sites are shown only if relevant. H, HindIII; S, SpeI.