TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide | Sequence | Introduced restriction site |
|---|---|---|
| CgΔN270 | TAACGCAGAGCACATCTTTC | |
| CgCEN2 | CGCGTAACTAATGATGCAAT | |
| CgΔN292 | GGGAGATCTTGATGTCTGTCACATG | BglII |
| CgΔN308 | GGGAGATCTATCAAAAACAATTGCA | BglII |
| CgCEN1R | TTGAGATCTTAACTAATGATGCAAT | BglII |
| CgΔIDelete | CAGTGATGTCTGTATCAAAAACAATTGC | |
| CgΔIRandom | CAGTGATGTCTGTGGATCCATCAAAAACAATTGC | |
| CgΔIExchange | CAGTGATGTCTAGCACATCTTCAAAAACAATTGGC | |
| CgCEN6 | CGGGATCCAGCACATCTACCGGATAGAGCGTAAG | BamHI |
| CgCEN6R | AAGGATCCTAACTAATGATGCAAT | BamHI |
| Cg1/1Ec | GGCCGGATCCATGGAGAGAAGCAACC | BamHI |
| Cg1/3Ec | GGTTGGATCCCTATTCCTTAGACTCATC | BamHI |
| Cg1/1A | CCCGGGTACCATGGCCCAACTTTG | KpnI |
| Cg1/1B | TTAACTCGAGCTCCTCCTTCTCATCG | XhoI |
| Cg1/2A | GGGCTCGAGTCTAAGGAATAGATA | XhoI |
| Cg1/2B | AAATCTAGAGCTGAGGTTCATGGA | XbaI |
| Cg1/3 | ACCATTAATGTTGGC | |
| Cg1/4 | AGTATCTTCAGGTAT | |
| Cg1/7 | TTAACTTGGCCATTGATA | MscI |
| Cg1/8 | CCTTGGCATATGTTCCTTAGACTCATCTTC | NdeI |
| Cg1/9 | GAAGATCTATATTGAAAATGATCTGGTAT | BglII |
| Cg1/10 | CCCAAGCTTGTTGGACAGAGAAAAATAG | HindIII |
| Cg1/11 | GGAATTCAGAATGATGGAGAGAAGCAACCGT | EcoRI |
| Cg1/12 | GGGGTACCAGGTTCGTGGCCCTGCTCCTG | KpnI |
| Cg1/13 | GCTCTAGATCTCAATGGACCACGACAACG | XbaI |
| Cg1/14 | GTACTAGTGGTATAAAGCT | SpeI |
| CgCDEI.F | CAGTGATGTCTGTCACATGATCAAA | |
| CgCDEI.R | CTACAGACAGTGTACTAGTTTGTCA | |
| CgACT1 | CGGGGTACCATGGATTCTGGTATGTTCGA | |
| CgACT4 | TATTCACACACGCAGGGACAG | |
| CgHis1 | AGCATGGCGGGTGGTCTC | |
| CgHis2 | CCAGCTCCACCACGGCGA | |
| HA-F | GGAATTCCATATGGTTGTTTACCCATACGAT | NdeI |
| HA-R | CCCAGATCTCTACTAATCTGGAACGTCATATGGATA | BglII |