TABLE 4.
Rates of plasmid loss observed with CDEI mutant centromeres determined by replica-plating assay
| Plasmid | No. of colonies on YPDa | No. of colonies on selective medium | Plasmid loss frequency (± SD)b | Relative loss frequencyc |
|---|---|---|---|---|
| pBM-WT | 428 (1) | 425 | 7 × 10−3 | 1 |
| PBM-Δ278-6/6R | 3,872 (3) | 1,790 | 5.4 (±0.6) × 10−1 | 77 |
| pBM-Δ308 | 1,858 (2) | 940 | 4.9 (±0.1) × 10−1 | 70 |
| pBM-ΔCEN | 2,608 (2) | 647 | 7.5 (±0.3) × 10−1 | 107 |
a
Numbers in parenthesese indicate the numbers of independent experiments whose results were summarized.
b
Number of colonies on YPD − number of colonies on selective medium/number of colonies on YPD.
c
Normalized to the loss frequency of the wild type.