Figure 3.

Adipocyte differentiation promotes End-MT and EPA-E inhibits its effect. (a) The 3T3-L1 cells were cultured in differentiation medium for 8 days. Cells that were differentiated into adipocytes were stained with Oil red O staining. Oil red O staining was evaluated by measuring the absorbance of the eluate. Bar = 100 μm. (b) and (c) Protein expression (b) and mRNA expression (c) of TGF-β in the 3T3L1 preadipocyte cells. (d) End-MT was evaluated using the Boyden chamber assay. ^∗P < 0.05. ^∗∗P < 0.01. These data are expressed as means ± SD.