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. 2022 Jan 1;25(1):103722. doi: 10.1016/j.isci.2021.103722

Figure 2.

Figure 2

SARS-CoV-2 infection and its proteins targeting promote mitochondrial fragmentation in human iPSC-cardiomyocytes

(A) Representative confocal images of Ad mitoGCaMP6 as a mitochondrial marker in iPSC-CMs before or after 24 h of SARS-CoV-2 infection. SARS-CoV-2 MOI 1. Bar graphs depict the analysis of mitochondrial shape parameters.

(B) Representative confocal images of MitoTracker Red as a mitochondrial marker in iPSC-CMs before or after 24 h of SARS-CoV-2 infection. SARS-CoV-2 MOI 1. Bar graphs depict the analysis of mitochondrial shape parameters.

(A and B) Data are presented as the mean ± SEM, n = 5 independent experiments. ∗∗p <0.01, ∗∗∗p <0.01, ∗∗∗∗p <0.0001.

(C) Ectopic expression of individual SARS-CoV-2 proteins in COS-7 Cells.

(D-H) Individual viral proteins were transiently transfected in COS-7 cells to visualize the intracellular localization of viral proteins tagged with mRFP (red) on a single-cell basis. Representative confocal images of live cells stained with ER (ER Tracker, blue) and mitochondrial markers (DHR123, green). Spatial overlap and intensity profiles demonstrate ER and mitochondrial localization of viral proteins.

(H) Table depicts subcellular localization of thirteen SARS-CoV-2 proteins.

(I) Analysis of mitochondrial phenotypes (length and area) in cells expressing SARS-CoV-2 proteins. Data are presented as mean ± SEM, n = 3–6 independent experiments. ∗∗∗∗p <0.0001.