Fig. 5.

Efflux of FITC-labeled dextrans of different sizes from L929sAhFas iGSDME cells during apoptosis-driven secondary necrosis. a–d Flow cytometry analysis of FITC-labeled dextran release in L929sAhFas iGSDME cells with (L929sAhFas iGSDME+) and without (L929sAhFas iGSDME−) doxycycline-induced GSDME expression upon treatment with anti-Fas. a–c Graphs representing the relative mean fluorescence intensity (rMFI, relative to the untreated SB− population) for increasing sizes of FITC-labeled dextrans a in the SB− population, b in the SB low+ population and c in the SB high+ population after 8 h treatment with anti-Fas. d Graph representing the relative mean fluorescence intensity (rMFI) of the total cell population (relative to the total untreated cell population) as a function of anti-Fas treatment. FD FITC-labeled dextran, LsFas L929sAhFas, NTC non-treatment control