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. 2021 Dec 15;106(1):261–271. doi: 10.1007/s00253-021-11728-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — SpyADI PEGylation and purification. A Coomassie-stained SDS-PAGE gel and B Western blot showing M = marker, ADI = purified unpegylated SpyADI, ADI-PEG = SpyADI after the PEGylation reaction; C Coomassie-stained SDS-PAGE gel of elution fractions of the anion exchange chromatography for the separation of SpyADI and SpyADI-PEG20, numbers above the lanes = NaCl concentration the elution started with in the respective fraction (each fraction covers a 14 mM NaCl concentration range), M = marker; D Coomassie-stained SDS-PAGE gel showing M = marker, P1 = pooled and concentrated fractions eluted with 160 to 240 mM NaCl, P2 = pooled and concentrated fractions eluted with 295 to 390 mM NaCl