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. 2021 Dec 15;106(1):261–271. doi: 10.1007/s00253-021-11728-7

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© The Author(s) 2021

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Fig. 2 — PEGylation efficiency and impact on activity. A Concentration dependent absorbance of TNBSA stained SpyADI and SpyADI-PEG20 (means of n = 5 biological replicates). As described by Holtsberg et al. (2002), the molar PEGylation rate was calculated as x = (1 − (slope PEG-ADI / slope ADI)) × 26, where 26 refers to the number of accessible primary amino groups of SpyADI. B Relative specific activity of SpyADI-PEG20 in comparison to native SpyADI, means with standard deviations of n = 4 biological replicates