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. 2021 Dec 15;106(1):261–271. doi: 10.1007/s00253-021-11728-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Antitumoral activity of SpyADI or SpyADI-PEG20. GBM cells were treated with 35 mU SpyADI or SpyADI-PEG20 for 72 h. Biomass was assessed by Calcein AM staining and absorbance measurement at 570 nm. Absorbance measured for untreated control cells was set to 100% and the other values were related, respectively. Shown are means and standard deviations of n ≥ 3 biological replicates, *p < 0.05, ***p < 0.001, two-way ANOVA