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. 2021 Dec 16;106(1):349–367. doi: 10.1007/s00253-021-11731-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Treatment variants. Curves are based on actual measurements of temperature and osmolality read in real time by thermometer and in collected samples by osmometer, respectively. Y axis: temperature [°C]; auxiliary Y axis: osmolality [Osm kg⁻¹], X axis: time [h]; 1) control variant, 2) ↓Temp variant, 3) ↑Osm variant, 4) ↓Temp↑Osm variant. Color code is explained in the legend. Given values are mean from biological quadruplicate ± SD