Table 1.
RNA-seq details, genetic identity and qRT-PCR parameters of selected candidate reference genes and target genes
| Gene name | Abbreviation | FPKM value | CV (%) | E-value | Identity (%) | Amplicon size (bp) | Correlation coefficient (R2) | PCR efficiency (%) |
|---|---|---|---|---|---|---|---|---|
| Proteasome | PSMA5 | 54.31 | 4.74 | 0 | 95.24 | 80 | 105.8 | 0.95 |
| RNA polymerase | RNAp | 49.16 | 3.51 | 0 | 94.55 | 80 | 120.6 | 0.97 |
| ADP ribosylation Factor 2 | ADPRF2 | 407.79 | 4.86 | 0 | 97.8 | 80 | 96.5 | 0.95 |
| Translational Initiation Factor 5A4 | IF5A4 | 418.10 | 3.29 | 0 | 95.24 | 80 | 102 | 0.95 |
| Profilin | – | 199.61 | 4.31 | 8e−167 | 93.43 | 80 | 115 | 0.92 |
| Small nuclear ribo-nucleoprotein G | SNRNPG | 165.36 | 5.56 | 1e−110 | 97.92 | 80 | 109.1 | 0.94 |
| 26 S protease regulatory subunit | 26 S | 127.06 | 3.71 | 0 | 94.22 | 80 | 123 | 0.95 |
| Aquaporin | PIP 2 | 902.86 | 4.88 | 0 | 95.05 | 80 | 122.4 | 0.98 |
| Translationally control tumour protein | TCTP | 164.26 | 5.14 | 0 | 94.8 | 80 | 111.1 | 0.99 |