Table 2.
Ranking and expression stability value (SD) of nine candidate reference genes in horse gram as calculated by delta-delta Ct
| Rank | All samples | HPK4 | HPKM 317 | |||
|---|---|---|---|---|---|---|
| Gene | Mean SD | Gene | Mean SD | Gene | Mean SD | |
| 1 | PSMA5 | 2.50 | PSMA5 | 2.54 | PSMA5 | 2.45 |
| 2 | PIP2 | 2.59 | PIP2 | 2.62 | Profilin | 2.49 |
| 3 | Profilin | 2.63 | Profilin | 2.76 | PIP2 | 2.51 |
| 4 | RNAp | 2.75 | RNAp | 2.87 | RNAp | 2.64 |
| 5 | 26S | 2.79 | ADPRF2 | 2.89 | 26S | 2.64 |
| 6 | ADPRF2 | 2.81 | 26S | 2.93 | SnRNPG | 2.66 |
| 7 | IF5A4 | 2.84 | IF5A4 | 2.98 | IF5A4 | 2.69 |
| 8 | SnRNPG | 2.85 | SnRNPG | 3.04 | ADPRF2 | 2.72 |
| 9 | TCTP | 2.89 | TCTP | 3.04 | TCTP | 2.75 |