Table 3.
Ranking and expression stability value (CV ± SD) of nine candidate reference genes in horse gram as calculated by BestKeeper
| Rank | All samples | HPK4 | HPKM 317 | |||
|---|---|---|---|---|---|---|
| Gene | CV ± SD | Gene | CV ± SD | Gene | CV ± SD | |
| 1 | PIP2 | 6.98 ± 2.29 | PIP2 | 7.56 ± 2.49 | PIP2 | 6.41 ± 2.10 |
| 2 | Profilin | 8.55 ± 2.40 | TCTP | 8.92 ± 2.80 | Profilin | 7.89 ± 2.23 |
| 3 | TCTP | 9.31 ± 2.89 | Profilin | 9.11 ± 2.53 | ADPRF2 | 8.11 ± 2.49 |
| 4 | IF5A4 | 9.68 ± 3.04 | 26S | 9.77 ± 2.85 | IF5A4 | 8.56 ± 2.70 |
| 5 | 26S | 9.73 ± 2.84 | IF5A4 | 10.72 ± 3.36 | TCTP | 9.70 ± 2.99 |
| 6 | ADPRF2 | 10.56 ± 3.15 | SnRNPG | 10.95 ± 3.37 | 26S | 9.73 ± 2.85 |
| 7 | RNAp | 10.64 ± 3.40 | RNAp | 11.23 ± 3.55 | RNAp | 9.95 ± 3.22 |
| 8 | SnRNPG | 11.43 ± 3.53 | ADPRF2 | 12.59 ± 3.67 | SnRNPG | 11.83 ± 3.67 |
| 9 | PSMA5 | 14.79 ± 4.61 | PSMA5 | 15.85 ± 4.89 | PSMA5 | 13.75 ± 4.33 |