Table 5.
Ranking and expression stability value (M) of nine candidate reference genes in horse gram as calculated by geNorm
Rank | All samples | HPK4 | HPKM 317 | |||
---|---|---|---|---|---|---|
Gene | M value | Gene | M value | Gene | M value | |
1 | TCTP | 0.146 | TCTP | 0.144 | Profilin | 0.139 |
2 | Profilin | 0.147 | SnRNPG | 0.151 | TCTP | 0.144 |
3 | IF5A4 | 0.163 | Profilin | 0.154 | 26S | 0.156 |
4 | 26S | 0.171 | IF5A4 | 0.168 | IF5A4 | 0.159 |
5 | SnRNPG | 0.172 | 26S | 0.185 | ADPRF2 | 0.161 |
6 | RNAp | 0.181 | ADPRF2 | 0.193 | RNAp | 0.167 |
7 | ADPRF2 | 0.182 | RNAp | 0.195 | SnRNPG | 0.188 |
8 | PIP2 | 0.222 | PIP2 | 0.239 | PIP2 | 0.207 |
9 | PSMA5 | 0.271 | PSMA5 | 0.317 | PSMA5 | 0.223 |