Table 6.
Ranking order of the genes as determined by RefFinder analysis
| Ranking order (Better-Good-Average) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Method | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
| All samples | |||||||||
| Delta Ct | Profilin | TCTP | 26S | IF5A 4 | SnRNPG | ADPRF 2 | RNAp | PIP2 | PSMA5 |
| BestKeeper | PIP 2 | Profilin | 26S | TCTP | IF5A 4 | ADPRF 2 | RNAp | SnRNPG | PSMA5 |
| Normfinder | Profilin | TCTP | 26S | IF5A 4 | SnRNPG | ADPRF 2 | RNAp | PIP2 | PSMA5 |
| geNorm | Profilin/TCTP | 26S | IF5A 4 | RNAp | SnRNPG | ADPRF2 | PIP2 | PSMA5 | |
| Comprehensive ranking | Profilin | TCTP | 26S | IF5A 4 | PIP 2 | SnRNPG | ADPRF2 | RNAp | PSMA5 |
| Geomean values | 1.19 | 2.00 | 3.00 | 4.23 | 4.76 | 5.89 | 6.24 | 6.44 | 9.00 |
| HPK 4 genotype | |||||||||
| Delta Ct | TCTP | SnRNPG | Profilin | IF5A4 | 26 S | ADPRF 2 | RNAp | PIP 2 | PSMA5 |
| BestKeeper | PIP2 | Profilin | TCTP | 26S | IF5A4 | SnRNPG | RNAp | ADPRF2 | PSMA5 |
| Normfinder | TCTP | SnRNPG | Profilin | IF5A4 | 26S | ADPRF 2 | RNAp | PIP 2 | PSMA5 |
| geNorm | Profilin/TCTP | SnRNPG | IF5A4 | 26 S | RNAp | ADPRF 2 | PIP 2 | PSMA5 | |
| Comprehensive ranking | TCTP | Profilin | SnRNPG | IF5A4 | 26 S | PIP2 | ADPRF 2 | RNAp | PSMA5 |
| Geomean values | 1.32 | 2.06 | 2.91 | 4.23 | 4.73 | 4.76 | 6.70 | 6.74 | 9.00 |
| HPKM 317 genotype | |||||||||
| Delta Ct | Profilin | TCTP | 26 S | ADPRF 2 | IF5A4 | RNAp | SnRNPG | PIP 2 | PSMA5 |
| BestKeeper | PIP 2 | Profilin | ADPRF 2 | IF5A4 | 26 S | TCTP | RNAp | SnRNP G | PSMA5 |
| Normfinder | Profilin | TCTP | 26 S | ADPRF 2 | IF5A4 | RNAp | SnRNP G | PIP 2 | PSMA5 |
| geNorm | Profilin/ADPRF 2 | IF5A4 | 26 S | TCTP | RNAp | SnRNPG | PIP 2 | PSMA5 | |
| Comprehensive ranking | Profilin | ADPRF 2 | TCTP | 26 S | IF5A4 | PIP 2 | RNAp | SnRNPG | PSMA5 |
| Geomean values | 1.19 | 2.63 | 3.31 | 3.66 | 4.16 | 4.76 | 6.24 | 7.24 | 9.00 |