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. Author manuscript; available in PMC: 2022 Jul 15.
Published in final edited form as: Mol Cell. 2021 Jun 30;81(14):2989–3006.e9. doi: 10.1016/j.molcel.2021.05.027

Figure 3. EXO5 Forms a Complex with BLM and RPA.

Figure 3.

(A) Western blots of Flag immunoprecipitation (IP) with noted antibodies. Anti-HA detected overexpressed HA-Flag-EXO5. Western blots of (B) BLM and (C) RPA1 IP with anti-HA antibody to detect IP HA-Flag-EXO5. (D) Colocalization of GFP-EXO5 with RPA1 or BLM foci after noted drug treatment. Scale bar: 5 μm. (E) EXO5 foci per cell measured by overexpressing GFP-EXO5 in BLM-depleted cells before drug treatment (representative EXO5 foci in inset, nuclei stained with blue DAPI). Each experiment, 50 cells were analyzed. Data are represented as mean ± SEM (** p<0.01). See Table S1.