FIGURE 3.

MICA*008 EVs are taken up more efficiently by NK cells. The NKL cell line was incubated for 3 h with 30 μg/ml of PKH26‐labelled sEVs or 20 μg/ml of PKH26‐labelled mEVs derived from ARK transfectants. The fluorescence of internalized EVs was evaluated by FACS analysis and measured as the percentage of PKH26⁺ cells. (a) One representative experiment is shown. (b) The mean of six independent experiments is shown. (c) NKL cells were pre‐treated for 1 h with EDTA (10 mM), EIPA (50 μM), dynasore (50 μM), nystatin (40 μg/ml) and then incubated for 3 h with PKH26‐labelled EVs. The fluorescence of internalized EVs was evaluated by FACS and measured as the percentage of PKH26 positive cells referred to untreated cells which were considered as 100%. Values reported represent the mean of at least three independent experiments