FIGURE 5.

MICA*008⁺ EVs are preferentially uptaken by leukocyte subpopulations expressing NKG2D. Human primary peripheral blood mononuclear cells (PBMC) were incubated for 3 h with PKH26‐labelled mEVs expressing or not MICA*008. (a) Representative dot plot of different PBMC populations, by gating on CD45⁺CD56⁺CD3^– for NK cells, on CD4⁺ and CD8⁺ after gating CD45⁺CD56^–CD3⁺ for T lymphocytes. Representative uptake of these populations is shown. Values reported in each plot represent the percentage of PKH26⁺ cells. (b) The mean of at least four independent experiments is shown. (c) FACS analysis of NKG2D expression on different leukocyte populations. A representative plot is shown