TABLE 2.
Oligonucleotides used in this study to create Rad27p substrates
| Primer | Size | Sequencea |
|---|---|---|
| Nicked and flap substrate primers | ||
| Dnick | 18-mer | 5′-GTAAAACGACGGCCAGTG |
| D6nt | 24-mer | 5′-TTCCAAGTAAAACGACGGCCAGTG |
| D15nt | 33-mer | 5′-TAGAGCTGTTTCCAAGTAAAACGACGGCCAGTG |
| T44 | 44-mer | 3′-GCGGTCCCAAAAGGGTCAGTGCTGGCATTTTGCTGCCGGTCACG |
| U26 | 26-mer | 5′-CGCCAGGGTTTTCCCAGTCACGACCA |
| U25 | 25-mer | 5′-CGCCAGGGTTTTCCCAGTCACGACC |
| U24 | 24-mer | 5′-CGCCAGGGTTTTCCCAGTCACGAC |
| Bubble substrate primers | ||
| Dbubble | 80-mer | 5′-GACTCTCGACTCACGTAGAGCTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAAGTAAAACGACGGCCAGTGCTACGAG |
| Tbubble | 66-mer | 3′-CTGAGAGCTGCTGAGAGCTGAGTGCATCTCGACAA............GCTGG.............CATTTTGCTGCCGGTCACGATGCTCG |
| Additional primer | ||
| T1 | 22-mer | 5′-CATTTTGCTGCCGGTCACGTCA |
a
Annealed residues are underlined, and unannealed residues are in boldface. Thick and thin underlines indicate upstream and downstream regions, respectively.