| Component (concentration) | Volume (μl/reaction) |
|---|---|
| ddH2O | 22 – 20 |
| OneTaq master mix (2x) | 25 |
| Forward primer mixture (10 μM) | 1 |
| Reverse primer mixture (10 μM) | 1 |
| cDNA template | 1 – 3 |
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