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. Author manuscript; available in PMC: 2022 Dec 10.
Published in final edited form as: Sci Immunol. 2021 Dec 10;6(66):eabl5842. doi: 10.1126/sciimmunol.abl5842

Fig. 2. Binding properties and specificity of isolated naive antibodies.

Fig. 2.

(A) ELISA binding heat map of 38 naive IgGs. Binding to wildtype SARS-CoV-2 RBD (SARS-2), ΔRBM, individual RBD glycan variants, circulating variants, related CoVs, hCoVs and polyreactive antigens. (B) Pearson correlation analysis of SARS-CoV-2 RBD affinities and HCDR3 length (IMGT) (n = 38). (C) ELISA EC50 values for IgGs with detectable SARS-CoV-2 RBD binding (n = 33) based on kappa or lambda gene usage. Red bars indicate the mean EC50 values. (D) Wildtype SARS-CoV-2 RBD ELISA EC50 values plotted against EC50 for B.1.1.7 RBD (E) B.1.351 RBD. (F) Proportion of SARS-CoV-2 RBD binders with detectable ELISA affinity for variants of concern (VOC) B.1.1.7, B.1.351, and B.1.617.2 RBDs. (G) ELISA EC50 values to related sarbecovirus RBDs displayed in decreasing order of paired-sequence identity.