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. Author manuscript; available in PMC: 2022 Dec 10.
Published in final edited form as: Sci Immunol. 2021 Dec 10;6(66):eabl5842. doi: 10.1126/sciimmunol.abl5842

Fig. 5. in vitro affinity-matured naive antibodies retain intrinsic specificity.

Fig. 5.

(A) Enrichment of ab090 parent (grey) and affinity matured (blue) libraires to 100nM SARS-CoV-2 or B.1.351 RBD using flow cytometry. (B) Fold enrichment in monovalent KD over ab090 parent for selected affinity matured progeny. (C) Kinetic analysis using biolayer interferometry (BLI) for ab090 parent and progeny Fabs to monomeric WT and variant RBDs. (D) Enrichment of ab072 parent (grey) and affinity matured (blue) libraires to 100nM SARS-CoV-2 or B.1.351 RBD using flow cytometry (E) Fold enrichment in monovalent KD over ab072 parent for selected affinity matured progeny. (F) Kinetic analysis using biolayer interferometry (BLI) for ab072 parent and progeny Fabs to monomeric WT and variant RBDs. Kinetic data are calculated though global fit of titration curves from four independent antigen concentrations.