Figure 2. Preparation of 1/5 serial dilutions for plating out virus.

Take 50 µl of virus stock after freeze-thawing and use to make a 1/25 virus dilution in D0 media. Take 250 µl of this stock and add to a further tube containing 1 ml D0 media. Continue until 6 dilutions have been prepared, up to 1/78125.