Table 2. Preparation of LipofectamineTM 2000 mixture (Mix 2) .
| Component | Volume (µl) | |
| Per well | 3 wells | |
| LipofectamineTM 2000 (Thermo Fisher Scientific) | 4 | 12 |
| D0 media | 196 | 588 |
| Total | 200 | 600 |
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