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Grading CD133 and PAS in acute tubular injury. A CD133 staining was graded as 0 in normal proximal tubules with intact brush border staining by PAS. B Mild scattered CD133 staining was graded as 1 + with mild reduced PAS staining. C Darker CD133 staining in more proximal tubules was graded as 2 + . D Strong and diffuse CD133 staining in a collapsing glomerulopathy was graded as 3 + . Magnifications at × 600 for A–D
