Prestin behavior alters over post-induction time. A) The magnitude of Vh change (ΔVh) increases in an exponential manner over time. Fitted exponential parameters: a= 12.6 mV, 1/b = 1.9 h (see Methods). R2:(correlation coefficient of variation): 0.89. B) Prestin charge density also increases in an exponential manner. Fitted exponential parameters: a= 20.1 fC/pF, 1/b = 14.9 h (see Methods). R2: 0.99. C) On the other hand, the Boltzmann parameter z, which increases very early following induction (Bian et al., 2010), is stable after 4 hr. Error bars are SEM.