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. Author manuscript; available in PMC: 2022 Jan 2.
Published in final edited form as: Cell Rep. 2021 May 4;35(5):109097. doi: 10.1016/j.celrep.2021.109097

Figure 1. Cloning and identification of mouse and human cardiac prestin cDNA.

Figure 1.

(A) The amplified, full-length cardiac prestin cDNA from mouse inner ear, atrial (ACM), and ventricular cardiomyocytes (VCM) was obtained using inner ear cDNA as a positive control. The expression level of prestin is higher in mouse ventricular compared with that of atrial myocytes. The full-length ORF of the same size (~2.2 kb) was obtained from ACM and VCM.

(B) Cloning of human cardiac prestin (~2.2 kb). NTC, no template control.

(C) Expression of prestin detected by single-cell qPCR. Slc26a6, a member of the same gene family as prestin, was used as a control (*p < 0.05).

(D) Photomicrograph from smFISH using probes specific for Slc26a5. Single-ventricular myocytes were isolated from WT and compared with Slc26a5−/−.

(E) Photomicrograph from smFISH using probes specific for Slc26a6. Cells were counterstained using monoclonal anti-α-actinin2 antibodies.