| Antibodies |
|
|
|
| Anti-Slc26a5 |
Santa Cruz Biotechnology |
sc-30163 (H-294); RRID: AB_2190499 |
| Anti-pholloidin-FITC |
Sigma-Aldrich |
P1951; RRID:AB_2315148 |
| Anti-GFP antibody |
Abcam |
Ab290; RRID:AB_303395 |
| Anti-α-actinin2 antibody |
Sigma-Aldrich |
A7811; RRID:AB_476766 |
| Cy™3 AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody |
Jackson ImmunoResearch Inc., |
AB_2338000; RRID:AB_2338000 |
| Rabbit anti-mouse IgG Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 555 |
ThermoFisher Scientific |
A27028; RRID:AB_2536091 |
| Goat anti-mouse secondary antibody, Rhodamine |
ThermoFisher Scientific |
31660; RRID:AB_228308 |
| Goat anti-rabbit IgG secondary antibody, Oregon Green 488 |
ThermoFisher Scientific |
O-11038; RRID:AB_2539798 |
|
| Chemicals |
|
|
|
| 2,3-Butanedione Monoxime (BDM.) |
Sigma-Aldrich |
B0753 |
| Blebbistatin |
Abcam. |
Ab120425 |
| Lipofectamine 2000 |
Thermo Fisher Scientific |
11668019 |
|
| Software |
|
|
|
| GraphPad Prism |
GraphPad Software |
https://www.graphpad.com
|
| Zeiss Zen Software |
Carl Zeiss |
https://www.zeiss.com/corporate/int/home.html
|
| Origin 6.1 Software |
OriginLab Corp. |
https://www.originlab.com
|
| BioRender |
BioRender |
https://biorender.com
|
| Lab Chart 6.0 software |
AD Instruments |
https://www.adinstruments.com
|
| pClamp10 software |
Molecular Devices, LLC |
https://support.moleculardevices.com/s/article/Spectranet-our-new-Customer-Care-Portal
|
| Imaris Cell Imaging Software |
Oxford Instruments |
https://imaris.oxinst.com/
|
|
| Experimental models: organisms/strains |
|
|
|
|
Slc26a5−/− knockout mouse model |
Previously generated (Liberman et al., 2002) |
N/A |
|
Slc26a5YFP/Y.F.P. (KI) mouse model |
Previously generated (Yamashita et al., 2015) |
N/A |
| C57BL/6J mice |
The Jackson Laboratory |
https://www.jax.org/
|
| SV-129 mice |
Taconic Biosciences |
https://www.taconic.com/
|
