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. 2021 Dec 26;2021:2915019. doi: 10.1155/2021/2915019

Figure 1.

Figure 1

RSL3 upregulated lipid ROS and downregulated key ferroptosis protein expression in glioblastoma cells. (a) CCK-8 assay examining U87 and U251 cell viability at different concentrations of RSL3 treatment for 24 h. (b) Lipid peroxidation analysis for U87 and U251 treated with 0.25 μM and 0.5 μM RSL3 for 24 h, respectively. (c) Western blot analysis of ATF4, xCT, GPX4, and HO-1 expression in U87 and U251 treated with 0.25 μM and 0.5 μM RSL3 for 24 h, respectively. Experiments were repeated thrice, and data is presented as the mean ± SD. ∗p < 0.05,  ∗∗p < 0.01, and∗∗∗p < 0.001.