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The NF-κB pathway was activated in RSL3-induced ferroptosis. (a) The protein levels of NF-κB pathway after RSL3 treatment. (b) Cellular localization of p65 was analyzed by immunofluorescence. Scale bar: 25 μm. The mRNA and protein levels of IL-1β were determined by RT-qPCR (c) and western blotting (d). U87 and U251 cells were treated with 0.25 μM and 0.5 μM RSL3 for 24 h, respectively. Experiments were repeated thrice, and data is presented as the mean ± SD. ∗p < 0.05; ∗∗p < 0.01.
