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GPX4 silencing did not induce ferroptosis unless the NF-κB pathway was activated simultaneously. (a) CCK-8 assay examining cell viability after siRNA (120 nM) treatment for 72 h. Data is presented as the mean ± SD. ∗p < 0.05, ∗∗p < 0.01, and∗∗∗p < 0.001. (b) Western blot analysis of ferroptosis-related protein expression after siRNA treatment. Experiments were performed thrice.
