Figure 6.

CGRP induced the activation of NF-κB and MAPK signaling pathways. (a, b) CGRP dose-dependently promoted the phosphorylation of MAPK (p38, ERK, and JNK). (c, d) NF-κB signaling pathway was also concentration-dependently activated by CGRP, and this activated effect was achieved through the phosphorylation of IκB-α. (e) Immunofluorescence results suggested that the nucleus translocation of p65 was increased when human NP cells were treated with CGRP. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, and ^∗∗∗∗p < 0.0001; ns: no significance. Scale bar = 100 μm.