Figure 9.

Evaluation of the therapeutic effects of Rimegepant on injury-induced IVDD in vivo. (a, b) Immunofluorescence results suggested that higher protein translation of MMP 3 and lower protein translation of aggrecan were observed in the injury group, whereas these effects were significantly restored after being treated by Rimegepant. (c, d) Cell apoptosis in vivo was also evaluated, and the expression of Cleaved-caspase 3 was enhanced in the injury group compared to the sham group, while in the injury+Rimegepant group, the protein translation of Cleaved-caspase 3 was ameliorated in comparison to the injury group. ^∗/#p < 0.05, ^∗∗/##p < 0.01, ^{∗∗∗/###}p < 0.001, and ^{∗∗∗∗/####}p < 0.0001; ns: no significance. Scale bar = 100 μm.